热烈祝贺郝晓蒙和罗明副研究员等撰写的研究论文“Mild photothermal-driven nanorobots forinfected wound healing through effective photodynamic therapy and wound microenvironment remodeling”被化学工程材料类top期刊Chemical Engineering Journal（2023年IF: 13.4，中国科学院SCI 一区期刊）接收！
光动力疗法（PDT）作为一种广谱的抗菌方法在治疗浅表生物膜感染疾病方面展现出巨大潜力。然而，生物膜的屏障作用和营造的缺氧微环境会严重阻碍PDT对细菌清除的效果。鉴于此，我们发展了一种光热驱动纳米机器人，能够在低强度光照下高效运动并增强PDT效果，穿透和破坏生物膜，重塑伤口微环境，从而加速伤口愈合。该纳米机器人由碗状的聚多巴胺纳米粒子组成，其表面依次用α-淀粉酶、过氧化氢酶和二氢卟吩E6修饰。凭借独特的碗状结构，纳米机器人可以在温和光照下有效地在各种生物介质中推进。结合α-淀粉酶的酶解能力，自驱动纳米机器人可以在10分钟内将生物膜内的穿透深度增加3倍。同时，它们可以通过将原位H2O2分解成O2来缓解乏氧，从而增强PDT对深层细菌的杀灭效果，使生物膜降解效率达到91%。此外，它们可以快速内化到巨噬细胞中并调节其表型，从而增强巨噬细胞的病原体吞噬和组织修复能力。这些协同作用使它们在生物膜感染的小鼠伤口模型中表现出卓越的治疗效果。综上，该研究提出了一种有望有效对抗细菌生物膜相关感染的策略。
原文摘要如下：Biofilms provide a stable microenvironment that not only fosters bacterial survival but also poses great challenges for bacterial eradication. Photodynamic therapy (PDT) is a broad-spectrum antibacterial method with great potential for treating superficial biofilm-infected diseases. However, the protective barrier and hypoxic microenvironment within biofilms severely impede the efficacy of PDT. Herein, we developed photothermal-driven nanorobots (denoted asCe6-ACPBNRs), capable of efficient locomotion and PDT under mild irradiation conditions, to penetrate and disrupt biofilms, remodel the wound microenvironment, and thus accelerate the healing process. The nanorobots consisted of bowl-shaped polydopamine nanoparticles, whose surfaces were sequentially modified with α-amylase, catalase, and chlorin e6 (Ce6). Due totheir distinctive bowl-shaped structure, the nanorobots can effectively propel within various biological media upon exposure to mild laser irradiation. Assisted by α-amylase, the self-propelled nanorobots can achieve a 3-fold increase in penetration depth within biofilms in 10 minutes. Simultaneously, they can alleviate hypoxia by decomposing in situ H2O2 into O2, thereby significantly enhancing the efficacy of PDT against deep-seated bacteria and achieving a biofilm degradation efficiency of 91%. Moreover, the Ce6-ACPBNRs can rapidly internalize into macrophages and modulate their phenotype, thereby enhancing their pathogen phagocytosis and tissue repair capabilities. These synergistic effects enable them to exhibit enhanced therapeutic efficacy in wound healing within a bacteria-infected wound mouse model. Therefore, this study presents a promising strategy for combating bacterial biofilm-associated infections.
